Preparative fractionation of sub-10-kbp DNA molecules plays an important role in second-generation sequencing.
Although the switchback principle is similar to what is used in PFGE, the device yields much faster separations owing to its sparse and regular sieving array. studied a three-dimensional (3D) nanopost array as an optimal separation matrix for DNA fragments over a few kbps under a DC electric field, resulting in similarly fast separations due to the sparse matrix performed continuous flow PFGE in a micromachined post array by applying pulsed electric fields of slightly unequal strengths.
Using this method, we demonstrate a high-resolution separation of 0.5, 1, 2, 5, and 10 kbp DNA fragments within 2 min.
During the separation, DNA fragments are also purified from other ionic species.
They are thus, theoretically, not particularly susceptible to partitioning during melting of silicate rocks.
Fractional crystallisation of felsic minerals changes the Sm/Nd ratio of the resultant materials.